![]() Because small amounts of mobile phase are employed for microbore LC-IR, less solvent must be removed to achieve mobile phase elimination. ![]() For example, the C–H stretching region of solute IR spectra can be accessed in reverse phase flow cell LC-IR measurements by employing deuterated solvents such as D 2O and CD 3CN as mobile phases. In addition, because small quantities of mobile phase are employed for microbore LC separations, expensive mobile phases that may not otherwise be practical can be employed. These features of microbore LC simplify both flow cell and mobile phase elimination approaches to LC-IR. In addition, optimal flow rates for microbore LC are significantly lower than for analytical scale LC. The quantity of mobile phase needed for a separation by microbore LC is much less than that required for analytical scale LC separations. White, in Encyclopedia of Spectroscopy and Spectrometry (Second Edition), 1999 Microbore LC-IRĪlthough most LC separations are conducted with analytical scale columns, microbore LC columns provide better resolution and are becoming increasingly popular.
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